human phospho kinase antibody array kit  (R&D Systems)

Journal: bioRxiv

Article Title: Attenuated CaV1.2-BK channel protein interaction in bipolar disorder

doi: 10.1101/2025.08.08.669447

Figure Lengend Snippet: ( a ) STRING database hits for the CACNA1C (CaV1.2) interactors highlighted KCNMA1 (coding for the pore-forming subunit of the big potassium (BK) channel; ( b ) Immunofluorescence comparison for the CaV1.2 and BK channel for HC versus BP excitatory iPSC-neurons shows no significant difference in average image intensity normalized to CaMKII α . Similar results were obtained by western blotting (data not shown); ( c ) In situ proximity ligation assay (PLA) marking the interaction between CaV1.2 and BK channel as the red puncta dots shows significant decrease in interaction for the BP condition. Each dot here represents one image average; ( d ) Whole cell patch clamp electrophysiology showed a more depolarized state for the BP neurons and also decreased outward currents. Each dot represents neuron.

Article Snippet: The primary antibodies used for human neuronal cultured cells included: CaMKII α (R&D systems, MAB5584), MAP2 (Sigma, M2320), GFAP (Dako, Z0334), GAD67 (Millipore, MAB5406), vGLUT1 (Abcam, ab72311), synapsin-1 (Millipore, AB1543), PSD-95 (ABcam, ab2723), BRN2 (Cell Signaling tech.

Techniques: Immunofluorescence, Comparison, Western Blot, In Situ, Proximity Ligation Assay, Patch Clamp

Journal: bioRxiv

Article Title: Attenuated CaV1.2-BK channel protein interaction in bipolar disorder

doi: 10.1101/2025.08.08.669447

Figure Lengend Snippet: ( a ) Behavior phase switch study paradigm schema. The mice are first tested at baseline behavior in week 0, followed by acute sleep deprivation and re-testing in week 4. This is followed by a long rest and re-testing in week 8; ( b ) CaMKII α -driven KCNMA1 knockout heterozygous (BK) mice were tested for the behavior phase switch with forced swim test and showed a ‘phase switch’ with sleep deprivation of 4 hours. Unoprostone (Uno) was able to rescue this switch in knockout mice. Notably, no significant difference in behavior was observed for the wild type (WT) mice; ( c ) Behavior phase switch was also observed when the BK mice performed sucrose splash test. Each dot represents one mouse.

Article Snippet: The primary antibodies used for human neuronal cultured cells included: CaMKII α (R&D systems, MAB5584), MAP2 (Sigma, M2320), GFAP (Dako, Z0334), GAD67 (Millipore, MAB5406), vGLUT1 (Abcam, ab72311), synapsin-1 (Millipore, AB1543), PSD-95 (ABcam, ab2723), BRN2 (Cell Signaling tech.

Techniques: Knock-Out

Journal: Antibodies

Article Title: Targeting CD44 and EpCAM with Antibody Dye Conjugates for the Photoimmunotherapy of Prostate Cancer

doi: 10.3390/antib14010005

Figure Lengend Snippet: Generation of the anti-CD44 and anti-EpCAM antibody dye conjugates. ( a ) SDS gel of the purified antibodies CD44 T120C/D265C and EpCAM T120C/D265C under reducing conditions; ( b ) SDS gel of the purified antibodies CD44 T120C/D265C and EpCAM T120C/D265C under non-reducing conditions; ( c ) SDS gels of the purified antibody dye conjugates CD44 T120C/D265C -WB692-CB2 and EpCAM T120C/D265C -WB692-CB2 under reducing and non-reducing conditions, and under white and red light.

Article Snippet: The membrane was blocked with 5% non-fat milk in 0.1% Tween-20/PBS (M-PBST) for 1 h, followed by incubation with mouse anti-human CD44 (#ab82529, Abcam, Cambridge, UK) or mouse anti-human EpCAM (#sc-25308, Santa Cruz Biotechnology, Dallas, TX, USA) as primary antibodies overnight at 4 °C in M-PBST.

Techniques: SDS-Gel, Purification

Journal: Antibodies

Article Title: Targeting CD44 and EpCAM with Antibody Dye Conjugates for the Photoimmunotherapy of Prostate Cancer

doi: 10.3390/antib14010005

Figure Lengend Snippet: Cell binding of the antibodies and antibody dye conjugates. ( a ) CD44 and EpCAM expression of the target cells. Concentration-dependent binding of the antibody CD44 T120C/D265C and the conjugate CD44 T120C/D265C -WB692-CB2 to ( b ) PC3-PSMA and ( c ) PC3-MM2 cells; ( d ) binding to CD44 negative CHO cells at a saturation concentration of 20.8 nM. Concentration-dependent binding of the antibody EpCAM T120C/D265C and the conjugate EpCAM T120C/D265C -WB692-CB2 to ( e ) PC3-PSMA and ( f ) PC3-MM2 cells; ( g ) binding to EpCAM negative CHO cells at a saturation concentration of 20.8 nM. Mean values ± SD of three independent experiments. Abbreviation: Kd, dissociation constant.

Article Snippet: The membrane was blocked with 5% non-fat milk in 0.1% Tween-20/PBS (M-PBST) for 1 h, followed by incubation with mouse anti-human CD44 (#ab82529, Abcam, Cambridge, UK) or mouse anti-human EpCAM (#sc-25308, Santa Cruz Biotechnology, Dallas, TX, USA) as primary antibodies overnight at 4 °C in M-PBST.

Techniques: Binding Assay, Expressing, Concentration Assay

Journal: Antibodies

Article Title: Targeting CD44 and EpCAM with Antibody Dye Conjugates for the Photoimmunotherapy of Prostate Cancer

doi: 10.3390/antib14010005

Figure Lengend Snippet: PIT of PC or PCSC-like cells with the conjugates CD44 T120C/D265C -WB692-CB2 and EpCAM T120C/D265C -WB692-CB2. ( a ) Viability of PC3-PSMA, ( b ) PC3-MM2 or ( c ) CHO cells 24 h after PIT incubated with 10 µg/mL CD44 T120C/D265C , 10 µg/mL CD44 T120C/D265C -WB692-CB2, free dye or medium (untreated control), and irradiation with a light dose of 32 J/cm 2 . Control cells were not irradiated (0 J/cm 2 ). Viability of ( d ) PC3-PSMA, ( e ) PC3-MM2 and ( f ) CHO cells 24 h after PIT incubated with 10 µg/mL EpCAM T120C/D265C , 10 µg/mL EpCAM T120C/D265C -WB692-CB2, free dye or medium (untreated control), and irradiated with a light dose of 32 J/cm 2 . Control cells were not irradiated (0 J/cm 2 ). Mean values ± SD of three independent biological experiments. Statistical analyses by Student’s t -test (unpaired, parametric with Welch’s correction).

Article Snippet: The membrane was blocked with 5% non-fat milk in 0.1% Tween-20/PBS (M-PBST) for 1 h, followed by incubation with mouse anti-human CD44 (#ab82529, Abcam, Cambridge, UK) or mouse anti-human EpCAM (#sc-25308, Santa Cruz Biotechnology, Dallas, TX, USA) as primary antibodies overnight at 4 °C in M-PBST.

Techniques: Incubation, Control, Irradiation

Journal: Antibodies

Article Title: Targeting CD44 and EpCAM with Antibody Dye Conjugates for the Photoimmunotherapy of Prostate Cancer

doi: 10.3390/antib14010005

Figure Lengend Snippet: Combined PIT of PC cells and PCSC-like cells. ( a ) Viability of PC3-PSMA cells and ( b ) PC3-MM2 cells 24 h after PIT with 10 µg/mL CD44 T120C/D265C -WB692-CB2 or EpCAM T120C/D265C -WB692-CB2 alone or in combination, and a light dose of 16 J/cm 2 . Mean values ± SD of three independent biological experiments. Statistical analyses were performed using one-way ANOVA, followed by Tukey’s multiple comparison test for post hoc analysis, with an adjusted significance level of p = 0.05.

Article Snippet: The membrane was blocked with 5% non-fat milk in 0.1% Tween-20/PBS (M-PBST) for 1 h, followed by incubation with mouse anti-human CD44 (#ab82529, Abcam, Cambridge, UK) or mouse anti-human EpCAM (#sc-25308, Santa Cruz Biotechnology, Dallas, TX, USA) as primary antibodies overnight at 4 °C in M-PBST.

Techniques: Comparison